Anti Stress Activity of Phytoecdysteroids Isolated from Aerial Part of Silene claviformis
Chemical Science International Journal,
Aims: The aim of present study was to isolate the phytoedysteroids from aerial part of Silene claviformis (Caryophyllaceae) and investigate their biological activity.
Place and Duration of Study: The investigation were carried out during 2019 and 2020 at laboratory of the chemistry of glycosides and department of the pharmacological and toxicology of Institute of the Chemistry of Plant Substances AS RUz, Tashkent, Uzbekistan.
Methodology: The phytoecdysteroids were isolated from aerial part of Silene claviformis using chromatographic methods. Thin-layer chromatography made on Silufol UV-254 and Merck plates, Fluka Analytical Germany, by spraying with alcohol solution of vanillin and heating for 1-2 minutes for 90-1000, UV lamp light at 254 nm and 365 nm. Their structures were confirmed by NMR and IR spectroscopy. Sum of phytoecdysteroids was administered at a dose of 10 mg/kg orally. The data obtained during the experiments were processed and analyzed by the method of variation statistics using the Student t-criterion.
Results: Silene claviformis contains 2-deoxyecdysterone (1), polypodine B (2), 20-hydroxyecdysone (3), ecdysterone-20,22-acetalisovaleric aldehyde (4),integristeron A (5),cyasterone (6), ecdysterone-20,22-acetalisovalerian (7), 2-deoxy-α-ecdysone (8). The compounds 2 and 6 are reported for the first time from this genus. The biological activity (stress-protective effect) of the mentioned phytoecdysteroids studied for the first time. These compounds were tested on male mice for their biological activities and results showed that, the adrenal gland mass increased in relation to the adrenal gland mass of intact animals by 42.6%, they showed a significant decrease in the content of ascorbic acid and cholesterol by 56.5 and 49.1%, respectively. A significant decrease in glycogen content (by 30.1%) and a noticeable activation of lipid peroxidation processes were noted in the liver, as indicated by an increase of 69.2% in the content of malondialdehyde in the organ.
Conclusion: This is the first study reporting an orally biological investigation for Silene claviformis. The sum of phytoecdysteroids showed potent stress-protective activity. The stress-protective effect of phytoecdysteroids was more pronounced in compared the eleutherococcus extract.
- Silene claviformis
- eleutherococcus extract
- stress-protective effect
How to Cite
Syrov VN. On adaptogenic properties of phytoecdysteroids. Proceeding of the Uzbek Academy of Science. 1996;11:61-64.
Volodin VV, Syrov VN, Khushbaktova ZA, Volodina S. Stress-protective action of the ecdysteroid containing preparation Serpisten. Theoritical and Applied Ecology. 2012;1:18-24.
Dinan L. A strategy for the identification of ecdysteroid receptor agonists and antagonists from plants. Eur. J. Entomol. 1995;92:271–283.
Bondarenko ON. Genus Silene L. – Smolevka. - In the book: Key to plants of Central Asia,Tashkent. 1971;253-277.
Atta EM, Nassar AA, Hasan NM, Hasan AR. New flavonoid glycoside and pharmacological activities of Pteranthus dichotomus J. Nat. Prod. 2013;7:69-79.
Jia Z, Koike K, Nikaido T. Major triterpenoid saponins from Saponaria officinalic. J Nat. Prod. 1998;61:1368-1373.
Vanhaecke M, Dyubankova N, Lescrinier E, Ende W. Metabolism of galactosyl-oligosaccharides in Stellaria media- Discovery of stellariose synthase, a novel type of galactosyltransferase. Phytochemistry. 2010;71:1095-1103.
Golea L, Benkhaled M, Lavaud C, Long Ch and Haba H. Phytochemical components and biological activities of Silene arenarioides. J. Nat. Prod. Res. 2017;31(23):2801-2805.
Karlson P, Burdette WB. Mode of Action of Ecdysones. In Invertebrate Endocrinology and Hormonal Heterophylly. Springer. 1974;43–54.
Zeleny J, Havelka J, Slama K. Hormonally mediated insect-plant relationships: Arthropod populations associated with ecdysteroid-containing plant Leuzea carthamoides (Asteraceae). Eur J. Entomol. 1997;94:183–198.
O’Connor JD. Ecdysteroid action at the molecular level. In Comprehensive Insect Physiology and Biochemistry and Pharmacology, 2nd Ed.; Kerkut GA, Gilbert LI, Eds.; Pergamon Press: Oxford, UK. 1984;8:85–98.
Thiem B, Kikowska M, Mali´nski MP, Kruszka D, Napierała M, Florek E. Ecdysteroids: Production in plant in vitro cultures. Phytochem. Rev. 2017;16:603–622.
Baltaev UA. Phytoecdysteroids: Structure, sources, and biosynthesis in plants. Russ. J. Bioorg. Chem. 2000;26:799–831.
Dinan L, Lafont R. Effects and applications of arthropod steroid hormones (ecdysteroids) in mammals. J. Endocrinol. 2006;1–8:191.
Kizelsztein P, Govorko D, Komarnytsky S, Evans A, Wang Z, Cefalu WT, Raskin I. 20-Hydroxyecdysone decreases weight and hyperglycemia in a diet-induced obesity mice model. Am. J. Physiol. Endoc. Metab. 2009;296:433–439.
Syrov VN, Khushbaktova Z. The influence of ecdysterone and saparal on functional, biochemical and morphological indicators of working capacity. Uzb. biol. Journal. 1988;3:61−65.
Chermnykh NS, Shimanovskiĭ NL, Shutko GV, Syrov VN. The effect of methandrostenolone and ecdysterone on the physical endurance of animals and protein metabolism in skeletal muscle. Farmacology. and toxicology.1988;6: 57−60.
Mirzaev YuR, Syrov VN. The effect of phytoecdysteroids on the sexual activity of male rats. Dokl. AN RUz. 1992;3:47−49.
Volodin VV, Syrov VN, Khushbaktova Z, Volodina S. Stress-protective action of the ecdysteroid containing preparation Serpisten. Theoritical and Applied Ecology. 2012;1:18-24.
Syrov VN, Kurmukov AG. On the tonic properties of ecdysterone isolated from safflower levzea. Dokl. Academy of Sciences of the Uzbek SSR. 1977;12: 27−30.
Lafont R. Ecdysteroids and related molecules in animals and plants. Archives of Insect Biochemistry and Physiology. 1997;35(1-2):3-20.
Olennikov DN, Kashchenko NI. Phytoecdysteroids from Silene jenissensis. Chem. Nat. Comp. 2017;53(6):1199-1201.
Dardymov IV. Ginseng and Eleutherococcus. (Mechanism of Biological Action). [in Russian], Moscow. 1976;184.
Brekhman II. Eleutherococcus. [in Russian], Leningrad. 1968;188.
Lo S, Russell JC, Taylor AW. Determination of glycogen in small tissue samples. J.App. Physiol. 1970;28(2):234–236.
Burov YuV, Zhukov VI. Methods for the selection of substances for the treatment of alcoholism. J.Chem.Pharm. 1979;5:42–50.
Ratakhina LV. Adaptogen activity of Urtica dioca leaf infusion. Plant resources. 1993; 1:44–49.
Yusupova UYu, Ramazonov NSh, Usmanov DA. Phytoecdysteroids from the Plant Dianthus helenae. Chem. Nat. Comp. 2019;55(2):393-394.
Sadykov Z, Saatov Z, Garcia M, Girault JP. Ecdysteroids from Silene claviformis. Chem. Nat. Comp. 2001;3:223-225.
Yusupova UYu, Ramazonov NSh, Usmanov DA. Phytoecdysteroids from the aerial part of Silene popovii. Chem. Nat. Comp. 2020; 56(3):562-563.
Girault JP, Bathori M, Varga E, Szendrei K and Lafont R. Isolation and identification of new ecdysteroids from the Caryophyllaceae. J. Nat. Prod. 1990;53(2): 279-293.
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