Antibacterial, Phytochemical and Preliminary Toxicity Profile Studies of N-hexane Chloroform, Ethyl Acetate and Methanol Extracts of Andira inermis Stem Bark (Leguminosea)

A. A. Isah — 2022-01-15

Phytochemical screening remains the most effective method for identifying and screening medically active components of plant. This research work studied the phytochemical screening, toxicity profile and antibacterial activity of the stem bark of Andira inermis using four different extracts by varying the polarity of the solvents; n-hexane, chloroform, ethyl acetateand methanol. The screening revealed the presence of alkaloids and carbohydrates in all extracts, tannins and saponins were not detected in chloroform and n-hexane extract, glycoside was also not detected in the chloroform extract. The toxicity profile in vivo studies using the Lorke’s method revealed no significance changes in the body weight of the albino rats, and the LD50 was higher than 5000mg/kg. However, there was a significant changes in behavior of the albino rats such as, fatigue, diarrhea, restlessness etc at 1600 mg/kg, 2900mg/kg and 5000mg/kg doses of the extracts respectively. Antimicrobial studies was evaluated with four (4) each of different bacteria and fungi. The results revealed ethylacetate and chloroform (moderately polar solvents) extracts to be more active against both bacteria and fungi but the methanol (highly polar) and hexane (highly non polar) extracts possessed less activities as compared with the other extracts at 15, 20, 30 and 40% concentrations.

Introducing of Thermodynamic Van’t Hoff Equation in Process of Dissociation of Benzoic Acid at Ordinary Temperature Range

Shiv Prakash Mishra — 2022-04-19

In article, we have reported the thermodynamic Van’t Hoff equation in study of dissociation process of solute benzoic acid into water solvent at ordinary temperature in between of 288 K to 318 K. At this temperature range, the value of dissociation constant (Kc) of that acid is being to found as -4.169, -4.045, -3.993, -3.885, -3.848 and -3.788, for given concentrations of NaCl (sodium chloride) volume as its six ionic strength of 0.00, 0.05, 0.10, 0.30, 0.40 and 0.50 M, respectively, which are titrimetrically analyzed with 0.05 M basic NaOH (sodium hydroxide) solution. The pH value of each solution is measured by using of digital pH-meter as well. Here, observation reported that the pH value of benzoic acid into water at that range of temperature is being inversely related to concentrations of NaCl. In graph, a value of benzoic acid with there ionic strength (I) is plotted versus equilibrium dissociation constant (Kc) at specific 298 K, have shown the dissociation of acid into water is increases as increase the ionic strength. But, comparatively, the graph for increasing temperature range to thermodynamic dissociation constant (Ka) have shown that the dissociation is not always increases as if increases temperature.

Chemical Science International Journal

Antibacterial, Phytochemical and Preliminary Toxicity Profile Studies of N-hexane Chloroform, Ethyl Acetate and Methanol Extracts of Andira inermis Stem Bark (Leguminosea)

Introducing of Thermodynamic Van’t Hoff Equation in Process of Dissociation of Benzoic Acid at Ordinary Temperature Range