Chemical Science International Journal

The global rise in multidrug-resistant pathogens has intensified discovery of novel antimicrobial agents with improved efficacy and structural diversity. The azo compounds bearing pharmacophores, have attracted significant interest due to their dual role in coloration and biological activity. The purpose of this study was to synthesize and evaluate the antimicrobial efficacy of a novel azo dye against human pathogenic microorganisms. The core aim was to assess whether this structurally tailored azo dye could serve as a potential candidate for antimicrobial drug development or not. The ampyrone was used to synthesize azo dye for present research work. The synthesised azo dye was then evaluated for antimicrobial activity against human pathogens with established antimicrobial resistance. The result revealed that most pronounced activity was observed against S. aureus MTCC 737 (18.3 ±0.8 mm) whereases least was for P. aeruginosa MTCC 424 (14.5 ±0.7 mm) at 100 µg/disc azo dye. Hence, this synthesized azo dye was exhibited promising antimicrobial activity against both bacterial and fungal pathogens. The study supports the need of continued exploration of azo dye derivatives in the search for effective multifunctional antimicrobial agents along with broader pharmacokinetics evaluations.