Kinetics and Entropy Production of Force-induced Oligomeric Enzyme Catalysis in a Single Trajectory: Effect of Multiple Substrates
Anita Gangopadhyay
Department of Physics, Santipur College, Santipur, Nadia 741404, West Bengal, India
Biswajit Das
S.N. Bose National Centre For Basic Sciences, Block-JD, Sector-III, Salt Lake, Kolkata 700098, India
Kinshuk Banerjee
Department of Chemistry, University of Calcutta, 92 A.P.C Road, Kolkata 700009, India
Gautam Gangopadhyay *
S.N. Bose National Centre For Basic Sciences, Block-JD, Sector-III, Salt Lake, Kolkata 700098, India
*Author to whom correspondence should be addressed.
Abstract
Here we have estimated the macroscopic entropy production for a mechanical force induced single oligomeric enzyme Kinetics with multiple substrate in a chemiostatic condition. This result is supported by the calculation of the single trajectory approach of forward and reverse path as a tool of stochastic thermodynamics. Through the approach for a particular reaction channel in the forward trajectory and the corresponding reverse reaction channel in the backward trajectory we explored the contribution of each and every sub-reactions or channels to the overall enzymatic processes and made the trajectory analysis of multiple kind of substrates. We have calculated the individual contribution of each sub-reaction in the overall medium entropy production which is exactly valid in the non-equilibrium steady state and it can give an insight about the mechanism of a complex chemical reaction in presence of an applied mechanical force on oligomeric enzyme kinetics.
Keywords: Oligomeric enzyme, multiple substrates, single trajectory, mechanical force induced kinetics